As we all know, the heart of the liquid chromatograph is the liquid chromatographic column, which is provided by different brands and manufacturers, with different models, product numbers and chromatographic fillers. Liquid chromatographic column is more expensive, so how to solve the failure of liquid chromatographic column? For example. What to do if the liquid chromatography column is blocked? Now, Chengdu Moore Scientific Instrument Co., Ltd. has gathered many netizens' answers. Edit the answer as follows:

 

What to do if the liquid chromatography column is blocked?

 

Best answer: the first reaction after the liquid chromatography column is blocked is the unstable pressure line

 

At this time, we should first find out what is blocking up. Liquid phase protection? Liquid chromatography column? So you have to check from the beginning to the end where it's blocked.

 

Troubleshooting method: do not shut down the workstation, first unscrew the pre column screw, and then look at the pressure under the condition of maintaining a certain flow rate. If it is normal, tighten the pre column screw. Open the other end of the column and see the pressure change as well.

 

Solution: if the column is blocked, it is always flushing the column, and acetonitrile has the largest elution force. For a night, if it's not serious, it should be about the same. The most serious situation is that there is no help. Replace the post. ~ ~

 

Question reflection: first of all, consider your sample filtration.

 

What to do if the liquid chromatography column is blocked?

 

1: Try to recoil the liquid chromatographic column. If the recoil is not good, try to clean the sieve plate in the front of the liquid chromatographic column by ultrasonic.

 

2: Let's see why the pressure of the liquid chromatography column is increased and the detection pressure is increased. Wash the column with isopropanol or methanol.

 

3: The liquid chromatographic column is blocked. First, analyze the sample you have recently made. Is it of large polarity or small polarity? Choose the solvent to wash the column. Maybe you used the buffer salt solution and didn't wash it clean. You should first wash it with 95% water for 2-3 hours, and then reduce the proportion of water. The last way is to reverse the column. However, this will reduce the column efficiency, and the later liquid chromatography column will always be used reversely!

 

4: If the above method still can't solve the problem of liquid chromatographic column blockage, it's like consulting relevant manufacturers or trying to replace a new chromatographic column: